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bmkm10 96 96 eg  (fluidigm)


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    fluidigm bmkm10 96 96 eg
    Bmkm10 96 96 Eg, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmk+m10/96%2E96+DNA+Binding+Dye+Sample%2FLoading+Kit/pmc12714903-142-1-4
    Average 94 stars, based on 5 article reviews
    bmkm10 96 96 eg - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Methods for diagnosing and treating inflammatory bowel disease
    Article Snippet: .. Real-time PCR reactions were run on the BioMarkTM HD System with Fluidigm pre-amp master mix (Life Technologies, Cat. No. 4391128) and reagents (Fluidigm, Cat. No. BMK-M10-96.96) using human CD3ε (Cat. No. Hs00167894_m1), αE (Cat. No. Hs01025372_ml), β7 (Cat. No. Hs01565750 m1), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cat. No. Hs99999905m1) primer sets (all from Applied Biosystems [Life Technologies]) according to manufacturer's instructions. ..

    Article Title: Methods for diagnosing and treating inflammatory bowel disease
    Article Snippet: .. Real-time PCR reactions were run on the BioMarkTM HD System with Fluidigm pre-amp master mix (Life Technologies, Cat. No. 4391128) and reagents (Fluidigm, Cat. No. BMK-M10-96.96) using human CD3ε (Cat. No. Hs00167894_m1), αE (Cat. No. Hs01025372_m1), (37 (Cat. No. Hs01565750_m1), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cat. No. Hs99999905_m1) primer sets (all from Applied Biosystems [Life Technologies]) according to manufacturer's instructions. ..

    Polymerase Chain Reaction:

    Article Title: Hematopoietic Stem Cell Heterogeneity Is Linked to the Initiation and Therapeutic Response of Myeloproliferative Neoplasms.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-CD34 BD Biosciences Cat# 555824 RRID:AB_398614 PE-Cy7 anti-CD38 BD Biosciences Cat# 555483 RRID:AB_395875 PerCP-Cy5.5 anti-CD123 BD Biosciences Cat# 560904 RRID:AB_10563250 APC-H7 anti-CD45RA BD Biosciences Cat# 560674 RRID:AB_1727497 APC anti-CD41 BD Biosciences Cat# 561852 RRID:AB_10895580 PE-CF594 anti-phosphorylated STAT3 BD Biosciences Cat# 562673 RRID:AB_2737714 Alexa Fluor 647 anti-phosphorylated STAT5 BD Biosciences Cat# 612599 RRID:AB_399882 eFluor 450 anti-phosphorylated STAT1 Thermo Fisher Scientific Cat# 48-9008-42 RRID: AB_2574119 BV421 anti-CD34 BioLegend Cat# 343610 RRID:AB_2561358 BV785 anti-CD38 BioLegend Cat# 303530 RRID:AB_2565893 BV605 anti-CD123 BioLegend Cat# 306026 RRID:AB_2563826 BV510 anti-CD41a BioLegend Cat# 303736 RRID:AB_2687213 FITC anti-VWF Abcam Cat# ab8822 RRID:AB_306799 PerCP anti-IFN-a/b R1 R and D Systems Cat# FAB245C RRID:AB_10972109 Alexa Fluor 700 anti-IFN-g R1/CD119 R and D Systems Cat# FAB6731N RRID:N/A Biological Samples Human bone marrow from MPN patients and normal controls Biobank of the Institute of Hematology and Blood Diseases Hospital N/A Human umbilical cord blood Institute of Hematology and Blood Diseases Hospital N/A Chemicals, Peptides, and Recombinant Proteins Histopaque-1077 Sigma-Aldrich Cat# 10771 IMDM Sigma-Aldrich Cat# I3390 StemSpan SFEM STEMCELL Technologies Cat# 09650 StemSpan CC100 STEMCELL Technologies Cat# 02690 Recombinant human IL-6 STEMCELL Technologies Cat# 78050.1 Recombinant human IL-3 Sigma-Aldrich Cat# I1646 Recombinant human SCF Prospec Bio Cat# CYT-255 Recombinant human TPO PeproTech Cat# 300-18 Recombinant human Flt3-Ligand PeproTech Cat# 300-19 Recombinant human IFNa Kaiyinyisheng Cat# S20030030 Recombinant human IFNg Thermo Fisher Scientific Cat# PHC4031 Phosphate Buffered Saline solution Hyclone Cat# SH30256.FS Fetal Bovine Serum GIBCO Cat# 16000-044 Penicillin Streptomycin GIBCO Cat# 15140-122 UltraPure 0.5M EDTA Invitrogen Cat# 15575-038 DAPI Sigma-Aldrich Cat# D9542 RNase Inhibitor Invitrogen Cat# AM2684 Triton X-100 Sigma-Aldrich Cat# T8787 dNTP Mix Thermo Fisher Scientific Cat# R0193 SuperScript II reverse transcriptase SuperScript II first-strand buffer DTT Invitrogen Cat# 18064-071 Betaine Sigma-Aldrich Cat# 61962 Magnesium chloride Sigma-Aldrich Cat# M8266 KAPA HiFi HotStart ReadyMix Kapa Biosystems Cat# KK2602 (Continued on next page) e1 Cell Stem Cell 28, 502–513.e1–e6, March 4, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beckman Coulter Cat# A63881 Dynabeads MyOne Streptavidin C1 Invitrogen Cat# 65001 TransTaq-T PCR SuperMix TransGen Biotech Cat# AS122-01 TaqMan SNP Genotyping Assay Thermo Fisher Scientific Cat# 4351374 TaqMan Gene Expression Master Mix Applied Biosystems Cat# 4369016 Critical Commercial Assays Lineage Cell Depletion Kit, human Miltenyi Biotec Cat# 130-092-211 CD34 MicroBead Kit, human Miltenyi Biotec Cat# 130-046-702 MegaCult-C Complete Kit with Cytokines for human CFU-Mk assays STEMCELL Technologies Cat# 04971 Transcription Factor Phospho Buffer Set BD Biosciences Cat# 563239 Zombie NIR viability kit BioLegend Cat# 423105 SMART-Seq Ultra Low Input RNA Kit TaKaRa Bio Cat# 634888 Advantage 2 PCR kit TaKaRa Bio Cat# 639206 NEBNext Ultra II DNA Library Prep Kit New England Biolabs Cat# E7645L DNA Clean & Concentrator Zymo Research Cat# D4014 Qubit dsDNA HS Assay Kit Invitrogen Cat# Q32854 Bioanalyzer High Sensitivity DNA Kit Agilent Cat# 5067-4626 KAPA Hyper Prep Kits with PCR Library Amplification Primer Mix Kapa Biosystems Cat# KK8504 CellsDirect One-Step qRT-PCR Kit (Superscript III RT mix; 2 3 Reaction mix) Invitrogen Cat# 11753-500 Single-cell PCR Sample/Loading Kit for gene expression Fluidigm Cat# BMK-M10-48.48 Deposited Data Raw and analyzed RNA-seq data This paper GEO: GSE111410 GSA: HRA000669 Oligonucleotides See Table S4 N/A Software and Algorithms FlowJo FlowJo https://www.flowjo.com GraphPad Prism GraphPad https://www.graphpad.com/ Seurat v3.0.2 Butler et al., 2018 https://satijalab.org/seurat/ clusterProfiler v3.10.1 Yu et al., 2012 https://guangchuangyu.github.io/software/clusterProfiler/ GSEA v4.0 Subramanian et al., 2005 https://www.gsea-msigdb.org/gsea/index.jsp Trimmomatic v0.36 Bolger et al., 2014 http://www.usadellab.org/cms/?page=trimmomatic Hisat2 v2.1.0 Kim et al., 2015 https://daehwankimlab.github.io/hisat2/ Stringtie software v1.3.3b Pertea et al., 2016 http://ccb.jhu.edu/software/stringtie/ R v3.6 R-Project https://www.r-project.org/ Picard N/A https://broadinstitute.github.io/picard/ Python v3.7 N/A https://www.python.org/ ..

    TaqMan SNP Genotyping Assay:

    Article Title: Hematopoietic Stem Cell Heterogeneity Is Linked to the Initiation and Therapeutic Response of Myeloproliferative Neoplasms.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-CD34 BD Biosciences Cat# 555824 RRID:AB_398614 PE-Cy7 anti-CD38 BD Biosciences Cat# 555483 RRID:AB_395875 PerCP-Cy5.5 anti-CD123 BD Biosciences Cat# 560904 RRID:AB_10563250 APC-H7 anti-CD45RA BD Biosciences Cat# 560674 RRID:AB_1727497 APC anti-CD41 BD Biosciences Cat# 561852 RRID:AB_10895580 PE-CF594 anti-phosphorylated STAT3 BD Biosciences Cat# 562673 RRID:AB_2737714 Alexa Fluor 647 anti-phosphorylated STAT5 BD Biosciences Cat# 612599 RRID:AB_399882 eFluor 450 anti-phosphorylated STAT1 Thermo Fisher Scientific Cat# 48-9008-42 RRID: AB_2574119 BV421 anti-CD34 BioLegend Cat# 343610 RRID:AB_2561358 BV785 anti-CD38 BioLegend Cat# 303530 RRID:AB_2565893 BV605 anti-CD123 BioLegend Cat# 306026 RRID:AB_2563826 BV510 anti-CD41a BioLegend Cat# 303736 RRID:AB_2687213 FITC anti-VWF Abcam Cat# ab8822 RRID:AB_306799 PerCP anti-IFN-a/b R1 R and D Systems Cat# FAB245C RRID:AB_10972109 Alexa Fluor 700 anti-IFN-g R1/CD119 R and D Systems Cat# FAB6731N RRID:N/A Biological Samples Human bone marrow from MPN patients and normal controls Biobank of the Institute of Hematology and Blood Diseases Hospital N/A Human umbilical cord blood Institute of Hematology and Blood Diseases Hospital N/A Chemicals, Peptides, and Recombinant Proteins Histopaque-1077 Sigma-Aldrich Cat# 10771 IMDM Sigma-Aldrich Cat# I3390 StemSpan SFEM STEMCELL Technologies Cat# 09650 StemSpan CC100 STEMCELL Technologies Cat# 02690 Recombinant human IL-6 STEMCELL Technologies Cat# 78050.1 Recombinant human IL-3 Sigma-Aldrich Cat# I1646 Recombinant human SCF Prospec Bio Cat# CYT-255 Recombinant human TPO PeproTech Cat# 300-18 Recombinant human Flt3-Ligand PeproTech Cat# 300-19 Recombinant human IFNa Kaiyinyisheng Cat# S20030030 Recombinant human IFNg Thermo Fisher Scientific Cat# PHC4031 Phosphate Buffered Saline solution Hyclone Cat# SH30256.FS Fetal Bovine Serum GIBCO Cat# 16000-044 Penicillin Streptomycin GIBCO Cat# 15140-122 UltraPure 0.5M EDTA Invitrogen Cat# 15575-038 DAPI Sigma-Aldrich Cat# D9542 RNase Inhibitor Invitrogen Cat# AM2684 Triton X-100 Sigma-Aldrich Cat# T8787 dNTP Mix Thermo Fisher Scientific Cat# R0193 SuperScript II reverse transcriptase SuperScript II first-strand buffer DTT Invitrogen Cat# 18064-071 Betaine Sigma-Aldrich Cat# 61962 Magnesium chloride Sigma-Aldrich Cat# M8266 KAPA HiFi HotStart ReadyMix Kapa Biosystems Cat# KK2602 (Continued on next page) e1 Cell Stem Cell 28, 502–513.e1–e6, March 4, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMPure XP Beckman Coulter Cat# A63881 Dynabeads MyOne Streptavidin C1 Invitrogen Cat# 65001 TransTaq-T PCR SuperMix TransGen Biotech Cat# AS122-01 TaqMan SNP Genotyping Assay Thermo Fisher Scientific Cat# 4351374 TaqMan Gene Expression Master Mix Applied Biosystems Cat# 4369016 Critical Commercial Assays Lineage Cell Depletion Kit, human Miltenyi Biotec Cat# 130-092-211 CD34 MicroBead Kit, human Miltenyi Biotec Cat# 130-046-702 MegaCult-C Complete Kit with Cytokines for human CFU-Mk assays STEMCELL Technologies Cat# 04971 Transcription Factor Phospho Buffer Set BD Biosciences Cat# 563239 Zombie NIR viability kit BioLegend Cat# 423105 SMART-Seq Ultra Low Input RNA Kit TaKaRa Bio Cat# 634888 Advantage 2 PCR kit TaKaRa Bio Cat# 639206 NEBNext Ultra II DNA Library Prep Kit New England Biolabs Cat# E7645L DNA Clean & Concentrator Zymo Research Cat# D4014 Qubit dsDNA HS Assay Kit Invitrogen Cat# Q32854 Bioanalyzer High Sensitivity DNA Kit Agilent Cat# 5067-4626 KAPA Hyper Prep Kits with PCR Library Amplification Primer Mix Kapa Biosystems Cat# KK8504 CellsDirect One-Step qRT-PCR Kit (Superscript III RT mix; 2 3 Reaction mix) Invitrogen Cat# 11753-500 Single-cell PCR Sample/Loading Kit for gene expression Fluidigm Cat# BMK-M10-48.48 Deposited Data Raw and analyzed RNA-seq data This paper GEO: GSE111410 GSA: HRA000669 Oligonucleotides See Table S4 N/A Software and Algorithms FlowJo FlowJo https://www.flowjo.com GraphPad Prism GraphPad https://www.graphpad.com/ Seurat v3.0.2 Butler et al., 2018 https://satijalab.org/seurat/ clusterProfiler v3.10.1 Yu et al., 2012 https://guangchuangyu.github.io/software/clusterProfiler/ GSEA v4.0 Subramanian et al., 2005 https://www.gsea-msigdb.org/gsea/index.jsp Trimmomatic v0.36 Bolger et al., 2014 http://www.usadellab.org/cms/?page=trimmomatic Hisat2 v2.1.0 Kim et al., 2015 https://daehwankimlab.github.io/hisat2/ Stringtie software v1.3.3b Pertea et al., 2016 http://ccb.jhu.edu/software/stringtie/ R v3.6 R-Project https://www.r-project.org/ Picard N/A https://broadinstitute.github.io/picard/ Python v3.7 N/A https://www.python.org/ ..



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